You handle liquid nitrogen? Remember to gear up!Visor, cryogenic gloves with cuffs, appropriate long-sleeved lab coat, overboots to wear over closed-toe shoes, and long pants without cuffs: this personal protective equipment is essential to protect yourself from the risks associated with handling liquid nitrogen.To learn the right reflexes, watch this short Inserm video!
https://www.youtube.com/watch?v=L30gFikywfw

Starting January 1, 2026, anyone who purchases or uses professional biocidal disinfectants must hold a Certibiocide certificate.
Without this individual certificate, both purchase and use will be illegal.
***At least one trained person per team/platform is required.***
The training lasts one day, is available online, and is valid for 5 years.
Contact your AP to find a suitable training.
A summary is available in this AP memo, along with the explanatory notice from the Ministry.
A few minutes of reading today, more safety tomorrow!


Pièces jointes

Thesis defense – Éloïse Daniel
Friday 28 November / 14:30

Venue : Centre Broca

Éloïse Daniel

Team : Planar polarity and plasticity
Neurocentre Magendie

Lieu : Centre Broca

Éloïse Daniel

Équipe : Polarité planaire et plasticité (Sans)
Neurocentre Magendie
Title

Identification and characterisation of new molecular tools to study the Planar Cell Polarity signalling and its role in cognitive processes
Abstract

A good memory is not just about correctly recalling learned information, but also about encoding memories distinctly from one another and being able to retrieve a complete memory from partial information. These two cognitive processes are pattern separation – or memory discrimination – and pattern completion – or memory generalisation. Both rely on the hippocampus, a brain structure that is crucial for learning, consolidating, and recalling memories. To accomplish these complex tasks, the hippocampus relies on the trisynaptic circuit composed of the entorhinal cortex, the dentate gyrus, and the CA3 region of the hippocampus. These structures can be compromised by alterations in intra- and intercellular signalling, either in adulthood or during normal and pathological ageing, or during the maturation of neural circuits. Numerous studies have shown that receptors, adhesion molecules, or scaffolding proteins may play a role in these memory processes.

Among these genes and proteins are members of the planar cell polarity (PCP) signalling pathway. This signalling pathway is conserved and known to shape tissues during development by regulating adhesion complexes and cytoskeletal dynamics. In this project, we are testing the hypothesis that modulation of PCP signalling disrupts the structural organisation of neurons in the dentate gyrus, which regulates specific cognitive processes such as pattern separation and pattern completion. We propose to characterise the role of C-Daam1 truncation – the C-terminal domain of the endogenous Daam1 protein belonging to PCP signalling – in specific brain functions. To do this, we combined molecular and cellular biology approaches with complex hippocampus-dependent behavioural tasks designed to capture the properties and elementary components of declarative memory in animals.

Our results show that overexpression of C-Daam1 in adult mice reduces the dendritic complexity of granule cells in the dentate gyrus and neuronal activation in both the dentate gyrus and CA3. Behaviourally, this translates into enhanced mnemonic discrimination (pattern separation) but impaired memory generalisation (pattern completion). In addition, PCP signalling dependent on Daam1 has recently been associated with synaptic dysfunction induced by β-amyloid accumulation, a protein directly involved in Alzheimer’s disease. We therefore evaluated the potential effect of C-Daam1 in transgenic mice modelling this pathology. Strikingly, C-Daam1 enables long-term memory maintenance in the early stages of the disease.

This project provides new insights into the mechanisms supporting functional memory and opens up new therapeutic avenues for maintaining mnemonic abilities during pathological ageing.

Keywords: Hippocampus, Memory, Planar Cell Polarity
Date de la soutenance: 28/11/2025 - 14h30
Lieu: Centre Broca

Pour plus de détails: https://www.bordeaux-neurocampus.fr/en/event/thesis-defense-eloise-daniel

The lab coat is much more than just work attire: it plays a key role in hygiene and safety. Check out the PDF to discover all the best practices. Stay in tune with the right measures, for safety without a false note!


Pièces jointes