Meeting room: Centre Broca

Program
9:00 – Jose A. ESTEBAN
Centro de Biología Molecular Severo Ochoa, Madrid
PI3K signaling for neuronal growth and synaptic plasticity

9:45 – Stéphane LÉON
Neurocentre Magendie (Cota Team)
Single-cell mapping of POMC neurons in obesity reveals novel subtypes with atypical identity

10:05 – Vincent PAGET-BLANC
IINS
A Synaptomic analysis reveals dopamine hub synapses in the mouse striatum

10:25-10:55 – Coffee break

10:55 – François LAMBERT
INCIA
Multimodal integration and plasticity in extraocular motoneurons: Synapse or phenotype matters?

11:25 – Ignacio FERNANDEZ MONCADA
Neurocentre Magendie (Marsicano Team)
CB1 receptors control memoryconsolidation via modulation of astroglial lactate and L-serine metabolism

11:45 – Agatha IDZIAK
IINS
It’s time for GreenT! A novel sensor for extracellular calcium imaging

12:05 – Cyril HANUS
Institute for Psychiatry and Neurosciences of Paris
N-glycans regulate synaptic receptor structure and function12:50-14:30 Lunch

12:50-14:30 – Lunch

14:30 – Anna BRACHET
IINS
Organization and role of beta spectrins in dendritic spines

15:00 – Lorena DELGADO
IMN
Normalization of substantia nigra pars reticulata parvalbumin-expressing neuron activity restores motor function in Parkinsonian mice

15:20 – Émilie PACARY
Neurocentre Magendie (Abrous Team)
The temporal origin of dentate granule neurons dictates their anatomical properties

15:50 – Nathan BENAC
IINS
Transient dopamine-NMDA receptor interaction governs hippocampal synaptic and network maturation

16:10 – Laura BALLERINI
SISSA, Trieste
Engineered nanomaterials to target synapses at the nanoscale

Scientific Committee
Elena AVIGNONE (Neurocentre Magendie)
Maurice GARRET (INCIA)
Mathieu LETELLIER (IINS)
Nathalie SANS (Neurocentre Magendie)

Registration
Please fill the registration formular by following the webpage down below:
https://framaforms.org/synapse-and-network-day-2022-1658751038

Presentations
Nora ABROUS, Neurocentre Magendie
11th July – Symposium 27 – Adult hippocampal neurogenesis in physiology and pathology
The temporal origin of dentate granule neurons dictates their morpho-functional properties

Laurent GROC, IINS
12th July – Symposium 35 – Antibodies in brain pathophysiology
Molecular interplay between autoantibodies and glutamate receptor at the plasma membrane

Serge AHMED, INCIA
> 13th July – Symposium 56 – Thinking outside the box: alternative targets and stratégies for addiction
Non-drug rewards as alternative to drug addiciton

Panelists in panel discussions
Wim CRUSIO, INCIA
> 9th July – Session 922
Stories form the home cage: an evening event focused on working with mice in the home cage

Anna BEYELER, Neurocentre Magendie
> 11th July – Session 920
ALBA-WWN-IBRO Mentoring Circle: Leadership and diversity at every step of the journey
> 12th July – Session 1280
Mentoring during the COVID-19 pandemic and beyond

The team « Adult neurogenesis and physiopathology » led by Dr Nora Abrous, at the Neurocentre Magendie INSERM U1215 in Bordeaux, is seeking for a highly motivated and talented young researcher for a 3 year postdoctoral contract to work on a FRM-funded project.

Project:

One research axis of Abrous lab is to better understand the differences/similarities between developmentally-born and adult-born granule neurons in the dentate gyrus of the hippocampus. The overarching goal of the FRM-funded project is to characterize the cellular, molecular and functional properties of the different cohorts of dentate granule neurons both under physiological condition and in response to adverse life events known to precipitate mental disorders. More specifically, the recruited postdoc will analyze the cellular (spines, axons, inputs, outputs…) and molecular (transcriptomic profile) characteristics of these different populations using a wide range of approaches: stereotaxic injections of virus, in vivo electroporation, transgenic mice, tissue clearing, confocal microscopy, RNAseq…

Candidate:

We are seeking for an enthusiastic candidate, with strong background in neuroscience and with expertise in RNAseq and bioinformatics. A theoretical background on hippocampal neurogenesis as well as previous experience with animal work and confocal microscopy will be valued.

Environment:

The successful candidate will work independently on his/her project, but interact closely with Dr Emilie Pacary and collaborate with other team members. He/She will be supported in his/her project by technical assistants for viral production, mouse breeding. The candidate will benefit from an excellent work environment, including various state-of–the art research facilities at Neurocentre Magendie (Genotyping, Transcriptomic…) and Bordeaux Neurocampus (Bordeaux Imaging Center, Bordeaux Bioinformatics Center…). Located in beautiful Bordeaux, the Neurocentre Magendie is one of the leading French centers in neuroscience research.

The position is available from 1st October 2022 and for 3 years.

Application:

Applications should include:

-A cover letter describing past research, research interest, career goals, and motivation to join our team
-A CV with the publication list
-Two references

Please submit your application to Emilie Pacary emilie.pacary@inserm.fr

Applications will be reviewed on a rolling basis until the position is filled.

Congratulations to the "Neurogenesis and Pathophysiology" team led by Nora Abrous, winner of the "FRM 2022 Team" award!

Presentation of the project
Link between the different phases of dentate gyrus development and stress-related pathologies

The mammalian brain is a complex organ made up of numerous regions interconnected by a very large number of neurons, and it is set up following a development that is both extremely precise and very finely regulated. Until about 30 years ago, it was taken for granted that this process only occurred during embryonic life, and that the adult brain did not give rise to any kind of neurogenesis. However, since then a growing body of evidence has demonstrated that in very scarce brain regions, new neurons can be generated throughout life. Among those, a structure called the dentate gyrus (DG) can be found, which itself belongs to the hippocampus. In fact, granular neurons (GNs), which make up the bulk of the GD architecture, are generated in several "waves" of development: the first occurs at the end of the embryonic period, and a second, the most abundant in terms of neurons generated, occurs a few days after birth. Thereafter, new NGs are produced during childhood, adolescence, adulthood and until the senescence of the individual. The GD thus appears to be an extremely heterogeneous structure, in which NGs assemblies of very different ontogenetic ages are mixed. Now, the GD is also a brain region known to be involved, both in memory and learning processes and in the sensitivity to stress.
We make the hypothesis that the temporal origin of a given population of NGs will determine their properties, and thus their functions and possible implications in the emergence of mental illnesses. In particular, those born during early development (embryonic and postnatal phases) would install a repertoire of rigid and basic behavioral responses, related to the most basic needs. Conversely, NGs born in adulthood would be able to sustain much more flexible behaviors, allowing the individual to adapt to the finest environmental changes. To demonstrate our hypothesis, we will use a range of innovative experimental models, ranging from cell biology to the most complex animal behaviors, such as tasks of similar context discrimination. This project will highlight the cellular diversity of the GD, and will open new avenues for understanding the ontogenetic origin of mental disorders, particularly those related to stress.

Nora Abrous, team leader and research director at Inserm (Unit 1215 "Neurocentre Magendie") was featured in the France 3 Nouvelle-Aquitaine news programme on 15 March.
Video: https://pod.inserm.fr/video/1434-nora-abrous_france-3_2022_03_15mkv/

Spotlight on a collaborative project involving teams from Magendie and IMN and supported by the FRM


Pièces jointes

Over the last 30 years, research has firmly established that active neurogenesis continues throughout adult life in discrete areas of the brain, such as in the hippocampal dentate gyrus (DG). This structure presents the peculiarity to exhibit a protracted development: dentate granule neurons (DGNs) are generated from the late embryonic period until adulthood and senescence with a maximal peak during the neonatal period. This continuous addition of DGNs and the resulting heterogeneity of the DG leads us to propose that the temporal origin of DGNs dictates their properties and functions. Supporting this view, we found that adult-born DGNs (Adu-DGNs) and DGNs born during adolescence (Ado-DGNs) are required for spatial learning but at different periods of animals’ life, whereas DGNs born during embryogenesis or early postnatally are not involved (Masachs et al., 2021).

The finding that new neurons are generated throughout life, humans included, has significant implications for brain repair. This has led to tremendous efforts to characterize how new neurons differentiate and integrate into adult neural circuitry. In this view, we have found a critical role for the atypical Rho GTPase Rnd2 in the survival, maturation, and function of adult-born DGNs (Kerloch et al., 2021). Our data has also revealed that Rnd2 has distinct functions during adult and developmental neurogenesis in the DG, arguing against the view that adult neurogenesis is a simple continuation of development.

Based on these observations and our previous work, we have proposed that developmentally-born DGNs establish and maintain a repertoire of basic and rigid behaviors. In contrast, adult-born DGNs sustained flexible behaviors to adapt to environmental changes (Abrous et al., 2021).

Meeting room: CGFB