Bordeaux School of Neurosciences:
The microscopic study of synapses relies on a large array of advanced techniques. Successful research in the field requires technological innovation and the interweaving of a variety of approaches at the molecular, cellular, and functional levels. The advanced course will allow the students to integrate the basic techniques in molecular and cellular neurobiology with advanced state-of-the art molecular, imaging and functional methodologies, through direct hands-on experiments. Taught techniques will range from proteomics and transcriptomics, in vitro and in vivo gene transfer (including viral technology), cellular imaging of proteins by confocal microscopy and by super-resolution microscopy (STED and PALM/STORM), single-particle tracking methodologies, live imaging of protein interactions (FRET, FLIM) at synapses, electron microscopy. The advanced course will also include electrophysiological and optophysiological approaches to address the functional properties of synaptic molecules such as recombinant receptors and to assess the role of various proteins in synaptic function and plasticity.
On-site chair: Nathalie Sans, PhD, University of Bordeaux, FR (Equipe Montcouquiol-Sans)