Lieu: Neurocentre Magendie Seminar room
"Implications of gabaergic miswiring on brain function: role of Nrg/Erbb4 signaling in interneuron development".
Invited by Andreas Frick
Abstract: Inhibitory cells are essential to fine-tuning the activity of neural circuits. Different classes of interneurons target distinct subcellular compartments of the pyramidal cell. This suggests that there is a differential contribution of each subtype of interneuron in the regulation of the network. In particular, cholecystokinin (CCK) expressing interneurons differ in chemical features and their postsynaptic targets, yet the role of each of these subtypes in the regulation of neuronal network activity is poorly understood. In this study, we describe a subpopulation of CCK interneurons co-expressing the vesicular glutamate transporter 3 (VGlut3) that also shows tyrosine kinase ErbB4 receptor expression. These interneurons are mainly basket cells targeting both, pyramidal cells and Parvalbumin-expressing interneurons in the hippocampus. To address the role of CCK-VGlut3 interneurons in network activity regulation, we combined mouse genetics with a set of different experimental approaches. Our results suggest that specific conditional deletion of ErbB4 receptor from CCK-expressing cells, results in deficient VGlut3 innervation. In addition, abnormal wiring of CCK-VGlut3 expressing interneurons perturbs cortical oscillatory activity as well as spatial representation in vivo and leads to specific behavioural deficits.