General informations
2015 Synapse Day Meeting

Promoters : Nathalie Sans (Neurocentre) & Maurice Garret (INCIA)

Magendie Speakers:
- Aude Panatier
- Guillaume Bony

Surface diffusion of astrocytic glutamate transporters shapes synaptic transmission. Murphy-Royal C, Dupuis JP, Varela JA, Panatier A, Pinson B, Baufreton J, Groc L, Oliet SH. Nat Neurosci. 2015 Feb;18(2):219-26. doi: 10.1038/nn.3901. Epub 2015 Jan 12.

Control of the glutamate time course in the synapse is crucial for excitatory transmission. This process is mainly ensured by astrocytic transporters, high expression of which is essential to compensate for their slow transport cycle. Although molecular mechanisms regulating transporter intracellular trafficking have been identified, the relationship between surface transporter dynamics and synaptic function remains unexplored. We found that GLT-1 transporters were highly mobile on rat astrocytes. Surface diffusion of GLT-1 was sensitive to neuronal and glial activities and was strongly reduced in the vicinity of glutamatergic synapses, favoring transporter retention. Notably, glutamate uncaging at synaptic sites increased GLT-1 diffusion, displacing transporters away from this compartment. Functionally, impairing GLT-1 membrane diffusion through cross-linking in vitro and in vivo slowed the kinetics of excitatory postsynaptic currents, indicative of a prolonged time course of synaptic glutamate. These data provide, to the best of our knowledge, the first evidence for a physiological role of GLT-1 surface diffusion in shaping synaptic transmission.

Feature produced in collaboration with Bertrand Nalpas, Research Director at Inserm and project manager Addiction - December 2014

Addictions are brain disease defined by an addiction to a substance or activity, with deleterious consequences. Researchers are trying to better describe the mechanisms involved in the development, maintenance and relapse of addiction. They also try to identify individual vulnerability factors, societal and environmental, for better prevention and care.

Article Neurosciences Bordeaux Neurocampus

Scribble1/AP2 Complex Coordinates NMDA Receptor Endocytic Recycling.
Piguel NH, Fievre S, Blanc JM, Carta M, Moreau MM, Moutin E, Pinheiro VL, Medina C, Ezan J, Lasvaux L, Loll F, Durand CM, Chang K, Petralia RS, Wenthold RJ, Stephenson FA, Vuillard L, Darbon H, Perroy J, Mulle C, Montcouquiol M, Racca C, Sans N. Cell Reports, Vol. 9, Issue 2, p712–727 Published online: October 9, 2014

Dans un travail publié en Octobre 2014 dans la revue Cell Reports, l’équipe “Polarite Planaire et Plasticite” dirigée par Mireille Montcouquiol et Nathalie Sans du Neurocentre Magendie (INSERM U862, Bordeaux) démontre le rôle clé des interactions entre la protéine de la polarité planaire Scribble1 et le complexe AP2 dans le maintien des récepteurs au glutamate de type NMDA au niveau des synapses.

General informations
Andreas Frick & al. in Nature Neuroscience

Dendritic channelopathies contribute to neocortical and sensory hyperexcitability in Fmr1−/y mice. Andreas Frick et al. Nature Neuroscience published online 10 November 2014; doi:10.1038/nn.3864 (voir les commentaires d'Andreas Frick)

Please also find below several articles, press releases, and radio interviews about this work:
1. Site Bordeaux Neurocampus
2. revue de presse Inserm
3. Radio Campus, direct link to mp3
4. Pour La Science
5. Sud Ouest
6. Les Echos
7. Le Point

Encoding of fear learning and memory in distributed neuronal circuits.Herry C, Johansen JP. Nat Neurosci. 2014 Dec;17(12):1644-1654. doi: 10.1038/nn.3869. Epub 2014 Nov 21. Review.Cyril Herry dans Nature neuroscience

General informations
Day 'Open Doors' of the technical platforms Magendie
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Friday 21th November 2014
From 12:30
Open to the entire scientific community

CONNECTOMICS : the wiring diagram of the human brain I September 22- 26, 2014

Stephane Oliet at the Neurocentre Magendie reviews extrasynaptic NMDA receptors organization structure and function in Philosophical Transactions of the Royal Society, another ‘bordelaise’ publication in the oldest scientific journal in the English-speaking world!

Authored by Dr. Thomas Papouin from Tufts University (Boston) and Dr. Stephane Oliet at the Neurocentre Magendie, the review discusses the most recent findings on extrasynaptic NMDA receptors and questions classical views regarding their structure, organization and function.