Neurocentre Magendie

Catherine BENNETAU




Enseignant-Chercheur

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Cursus:
Doctorat de 3ème cycle, 1988, Univ Bordeaux 1.
Doctorat d'Université, 1990, Univ Bordeaux 1.
Post-Doc 1990-1992 Brunel University,UK.
HDR 1998 Univ. Bordeaux 1.
PR1 Bordeaux Sciences Agro ex Enitab

Expertise: Nutrition, Estrogens, SERM, Receptors, Behavior, Memory



Physiologie animale, Biologiste cellulaire et moléculaire, Etude Clinique, Biodisponibilité, Pharmacocinétique, ELISA, Estrogènes, Isoflavones, SERM, Nutrition Humaine

 

Les estrogènes sont à la fois neurotrophiques et neuroprotecteurs. Leurs effets sont ubiquitaires et complexes sur l’ensemble des types cellulaires cérébraux passant par des voies de signalisation génomiques d’origine nucléaire ou membranaire mais aussi par des voies non génomiques rapides. Leur élucidation est incomplète à ce jour même si certaines des voies de signalisation mises en jeu sont impliquées dans la plasticité synaptique elle-même jouant un rôle dans les processus mnésiques. Certaines molécules d’origine alimentaire présentent des effets estrogéniques incomplets. On les désigne sous le nom de SERM (Selected Estrogen Receptor Modulators).
Nous nous proposons d’étudier les effets neuroprotecteurs des estrogènes sur la prévention de l’altération de la mémoire déclarative liée à l’âge. Ceci nous permettra ensuite d’identifier des molécules alimentaires présentant les effets bénéfiques des estrogènes sur la mémoire sans leurs effets néfastes sur les tissus périphériques. Notre approche est à la fois comportementale, cellulaire et biochimique.



40 publication(s) depuis Août 1995:


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Les IF indiqués ont été collectés par le Web of Sciences en


01/11/2016 | Food Chem   IF 4.1
Removing isoflavones from modern soyfood: Why and how?
Fernandez-Lopez A, Lamothe V, Delample M, Denayrolles M, Bennetau-Pelissero C

Abstract:
Estrogenic isoflavones were found, in the 1940s, to disrupt ewe reproduction and were identified in soy-consumers' urine in 1982. This led to controversy about their safety, often supported by current Asian diet measurements, but not by historical data. Traditional Asian recipes of soy were tested while assaying soy glycosilated isoflavones. As these compounds are water-soluble, their concentration is reduced by soaking. Pre-cooking or simmering time-dependently reduces the isoflavone:protein ratio in Tofu. Cooking soy-juice for 15 or 60min decreases the isoflavone:protein ratios in Tofu from 6.90 to 3.57 and 1.80, respectively (p<0.001). Traditional Tempeh contains only 18.07% of the original soybean isoflavones (p<0.001). Soy-juice isoflavones were reduced by ultra-filtration (6.54 vs 1.24 isoflavone:protein; p<0.001). Soy-protein and isoflavones are dissociated by water rinsing and prolonged cooking, but these have no equivalent in modern processes. As regards human health, a precise definition of the safety level of isoflavone intake requires additional studies.




21/03/2016 | Psychoneuroendocrinology   IF 4.2
Estradiol enhances retention but not organization of hippocampus-dependent memory in intact male mice.
Al Abed AS, Sellami A, Brayda-Bruno L, Lamothe V, Nogues X, Potier M, Bennetau-Pelissero C, Marighetto A

Abstract:
Because estrogens have mostly been studied in gonadectomized females, effects of chronic exposure to environmental estrogens in the general population are underestimated. Estrogens can enhance hippocampus-dependent memory through the modulation of information storage. However, declarative memory, the hippocampus-dependent memory of facts and events, demands more than abilities to retain information. Specifically, memory of repetitive events of everyday life such as 'where I parked' requires abilities to organize/update memories to prevent proactive interference from similar memories of previous 'parking events'. Whether such organizational processes are estrogen-sensitive is unknown. We here studied, in intact young and aged adult mice, drinking-water (1muM) estradiol effects on both retention and organizational components of hippocampus-dependent memory, using a radial-maze task of everyday-like memory. Demand on retention vs organization was manipulated by varying the time-interval separating repetitions of similar events. Estradiol increased performance in young and aged mice under minimized organizational demand, but failed to improve the age-associated memory impairment and diminished performance in young mice under high organizational demand. In fact, estradiol prolonged mnemonic retention of successive events without improving organization abilities, hence resulted in more proactive interference from irrelevant memories. c-Fos imaging of testing-induced brain activations showed that the deterioration of young memory was associated with dentate gyrus dysconnectivity, reminiscent of that seen in aged mice. Our findings support the view that estradiol is promnesic but also reveal that such property can paradoxically impair memory. These findings have important outcomes regarding health issues relative to the impact of environmental estrogens in the general population.




26/12/2014 | clin nutr   IF 4.5
Marked antioxidant effect of orange juice intake and its phytomicronutrients in a preliminary randomized cross-over trial on mild hypercholesterolemic men.
Constans J, Bennetau-Pelissero C, Martin JF, Rock E, Mazur A, Bedel A, Morand C, Berard AM

Abstract:
BACKGROUND & AIMS: Blond orange juice is the most consumed fruit juice in the world. It is a source of hesperidin, a bioavailable flavonoid reported to exhibit potential vascular protective actions. However, the specific impact on vascular function of Citrus phytomicronutrients, is unknown. For the first time, we investigated the effects of blond orange juice compared with a control beverage mimicking the composition of orange juice (including Vitamin C but no phytomicronutrients), on antioxidant markers, cardiovascular risk factors and endothelial function. METHODS: Twenty five male volunteers with two cardiovascular risk factors (age over 50 years and LDL-cholesterol between 130 and 190 mg/L) were enrolled in a randomized cross-over study. They received 3 times daily 200 mL of either blond orange juice or control beverage for 4 weeks, spaced by a 5-week wash-out. Endothelial function (flow mediated dilatation and plasma markers), oxidative status, lipid profile and inflammatory markers were assessed. RESULTS: Daily intakes of orange juice significantly led to a marked antioxidant effect which was correlated to hesperetin plasma levels and related with a decrease in reactive oxygen species. A tendency towards reduction of endothelial dysfunction and modest increase in plasma apoA-I concentration were also observed. This allows further experiments demonstrating the specific effect of phytomicronutrients from orange juice. CONCLUSIONS: These findings suggest that daily intake of nutritionally relevant dose of blond orange juice may contribute for a significant antioxidant effect through the phytochemicals contained in. Orange juice may be associated to other healthy foods to achieve a significant effect on the vascular function. This study is recorded in ClinicalTrials.com as NCT00539916.




02/2014 | Talanta   IF 4
Design and validation of a novel immunological test for enterolactone.
Shinkaruk S, Pinot E, Lamothe V, Schmitter JM, Baguenard L, Bennetau B, Bennetau-Pelissero C

Abstract:
Enterolactone (ENL) is produced by the gut microflora from lignans found in edible plants. ENL is estrogenic with no effect on the E-screen test and is a natural Selected Estrogen Receptor Modulator (SERM) with health interests that have to be checked in clinical studies with bioavailability assessment. Two haptens of ENL were synthesized, with a spacer arm at the C5 position having either 2 or 4 carbon atoms (ENLDelta2 and ENLDelta4, respectively). Hapten coupling to bovine serum albumin (BSA) was characterized by MALDI mass spectrometry. Polyclonal antibodies were obtained against the BSA conjugates. Additional conjugates were generated by coupling to swine thyroglobulin (Thyr). Homologous and heterologous competitive ELISAs were developed with Thyr or BSA conjugates as coating. The best assays were validated on biological samples from mice. Both antibodies exhibited the same IC50 at 1.5 ng mL(-1) with a detection limit below 0.5 ng mL(-1). Most cross-reactions with structurally related lignans were lower than 0.03%. This new assay type is faster, more specific and more reliable than existing ones.




06/2013 | anim reprod sci   IF 1.4
Reproductive impairment and endocrine disruption in goldfish by feeding diets containing soybean meal.
Bagheri T, Imanpoor MR, Jafari V, Bennetau-Pelissero C

Abstract:
A long-term feeding experiment was conducted to investigate the inclusion of soybean meal in diets for goldfish (Carassius auratus) on fish reproduction. In the present study, 20 weeks after hatching, goldfish with an initial average weight of 2+/-0.03g (mean+/-SD) were divided into 12 groups (three tanks per dietary treatment) and fed 400gkg(-1) crude protein diets. The four experimental diets were as follows: diet 1, fish meal (FM); diet 2, 35% soybean meal (SBM35%); diet 3, 65% soybean meal (SBM65%); diet 4, 100% soybean meal (SBM100%). After feeding with experimental diets, the impact on reproduction was investigated. In both males and females, the plasma testosterone (T) was significantly decreased, while 17beta-estradiol (E2) levels were significantly increased. Levels of 17alpha, hydroxyprogesterone. (17-OH-P) did not differ as a result of soybean meal feeding in either males or females. The average number of eggs spawned and sperm quality were reduced on feeding with soybean inclusion. Histological examination showed impact on oocyte maturation progress and spermatogenesis process in female and male fish, respectively. In addition, feeding goldfish with soybean meal until maturation caused reduction in fertilization and hatching rates in parallel to increasing soybean meal inclusion. The results demonstrated that inclusion of soybean meal might cause sex hormone biosynthesis disruption and reproductive impairments in fish, ultimately decreased fertilization as well as hatching rates in the offspring.




05/04/2013 | j proteome res   IF 4.2
Mass spectrometry-based metabolomics for the discovery of biomarkers of fruit and vegetable intake: citrus fruit as a case study.
Pujos-Guillot E, Hubert J, Martin JF, Lyan B, Quintana M, Claude S, Chabanas B, Rothwell JA, Bennetau-Pelissero C, Scalbert A, Comte B, Hercberg S, Morand C, Galan P, Manach C

Abstract:
Elucidation of the relationships between genotype, diet, and health requires accurate dietary assessment. In intervention and epidemiological studies, dietary assessment usually relies on questionnaires, which are susceptible to recall bias. An alternative approach is to quantify biomarkers of intake in biofluids, but few such markers have been validated so far. Here we describe the use of metabolomics for the discovery of nutritional biomarkers, using citrus fruits as a case study. Three study designs were compared. Urinary metabolomes were profiled for volunteers that had (a) consumed an acute dose of orange or grapefruit juice, (b) consumed orange juice regularly for one month, and (c) reported high or low consumption of citrus products for a large cohort study. Some signals were found to reflect citrus consumption in all three studies. Proline betaine and flavanone glucuronides were identified as known biomarkers, but various other biomarkers were revealed. Further, many signals that increased after citrus intake in the acute study were not sensitive enough to discriminate high and low citrus consumers in the cohort study. We propose that urine profiling of cohort subjects stratified by consumption is an effective strategy for discovery of sensitive biomarkers of consumption for a wide range of foods.




21/01/2013 | Br J Nutr   IF 3.4
Flavanone metabolites decrease monocyte adhesion to TNF-α-activated endothelial cells by modulating expression of atherosclerosis-related genes.
Chanet A, Milenkovic D, Claude S, Maier JA, Khan MK, Rakotomanomana N, Shinkaruk S, Bérard AM, Bennetau-Pelissero C, Mazur A, Morand C.

Abstract:
Flavanones are found specifically and abundantly in citrus fruits. Their beneficial effect on vascular function is well documented. However, little is known about their cellular and molecular mechanisms of action in vascular cells. The goal of the present study was to identify the impact of flavanone metabolites on endothelial cells and decipher the underlying molecular mechanisms of action. We investigated the impact of naringenin and hesperetin metabolites at 0·5, 2 and 10 &#956;m on monocyte adhesion to TNF-&#945;-activated human umbilical vein endothelial cells (HUVEC) and on gene expression. Except hesperetin-7-glucuronide and naringenin-7-glucuronide (N7G), when present at 2 &#956;m, flavanone metabolites (hesperetin-3'-sulphate, hesperetin-3'-glucuronide and naringenin-4'-glucuronide (N4'G)) significantly attenuated monocyte adhesion to TNF-&#945;-activated HUVEC. Exposure of both monocytes and HUVEC to N4'G and N7G at 2 &#956;m resulted in a higher inhibitory effect on monocyte adhesion. Gene expression analysis, using TaqMan Low-Density Array, revealed that flavanone metabolites modulated the expression of genes involved in atherogenesis, such as those involved in inflammation, cell adhesion and cytoskeletal organisation. In conclusion, physiologically relevant concentrations of flavanone metabolites reduce monocyte adhesion to TNF-&#945;-stimulated endothelial cells by affecting the expression of related genes. This provides a potential explanation for the vasculoprotective effects of flavanones.




01/12/2012 | Food Chem   IF 4.1
Bioavailability of glycitein relatively to other soy isoflavones in healthy young Caucasian men.
Shinkaruk S, Durand M, Lamothe V, Carpaye A, Martinet A, Chantre P, Vergne S, Nogues X, Moore N, Bennetau-Pelissero C

Abstract:
Glycitein is a Selective Estradiol Receptor Modulator (SERM) from soy. The study reports plasma bioavailability and urine excretion of glycitein compared to other soy isoflavones after a unique intake of food supplement based on soy germ containing 55.24mg isoflavones. Eighteen plasma and urinary sampling profiles collected over 48h from healthy young Caucasian men were analysed using specific ELISAs. Eight profiles contained equol. Glycitein T(max), C(max), AUC(0-->24h) and T((1/2)) in plasma were calculated. Urine T(max), % of excretion at 24h and clearance were assessed. Glycitein is one of the best absorbed flavonoids. Plasma steady-state level can be achieved by several intakes a day. Glycitein bioavailability is similar to that of daidzein and its urinary excretion is significantly higher than that of genistein. Equol does not affect glycitein bioavailability. Knowing glycitein bioavailability in man is essential for the development of soy-germ-based food supplements for health applications.




05/2012 | J Nutr Biochem   IF 4.7
Naringin, the major grapefruit flavonoid, specifically affects atherosclerosis development in diet-induced hypercholesterolemia in mice.
Chanet A, Milenkovic D, Deval C, Potier M, Constans J, Mazur A, Bennetau-Pelissero C, Morand C, Berard AM

Abstract:
Naringin (NAR) from grapefruit has exhibited potential protective effects against atherosclerosis development. However, specific mechanisms responsible for such effects are poorly understood. Thus, we aimed to investigate the antiatherogenic effects of NAR in different mouse models of hypercholesterolemia and decipher its molecular targets in the aorta using transcriptomic approach. Two mouse models of hypercholesterolemia, wild-type mice fed a high-fat/high-cholesterol diet and apolipoprotein E-deficient mice fed a semisynthetic diet, were studied. Mice were fed a respective control diets supplemented or not for 18 weeks with 0.02% of NAR, that is, nutritional supplementation. NAR supplementation reduced plaque progression only in wild-type mice fed the high-fat/high-cholesterol diet (-41%). Consistent with this protective effect, NAR reduced plasma non-high-density lipoprotein cholesterol concentrations as well as biomarkers of endothelial dysfunction. Microarray studies performed on aortas demonstrated differentially expressed genes encoding proteins involved in cell adhesion, actin cytoskeleton organization and cell division. Thus, the changes in gene expression induced by NAR could suggest a limited atherosclerosis progression by preventing immune cell adhesion and infiltration in the intima of vascular wall, as well as smooth muscle cell proliferation. Furthermore, this hypothesis was strengthened by in vitro experiments, which showed the ability of naringenin to reduce monocyte adhesion to endothelial cells and smooth muscle cell proliferation. In conclusion, this study revealed the antiatherogenic effect of NAR supplemented at a nutritionally achievable dose, specifically toward diet-induced atherosclerosis, and depicted its multitarget mode of action at the vascular level.




Abstract:
Competitive inhibition serological assay for detection of the phytoestrogen glycitein (Glyc) was developed using surface plasmon resonance (SPR) technique with protein conjugates and polyclonal antibodies initially designed for the enzyme-linked immunosorbent assays (ELISA). The efficiency of the approach to the quantification of the soy isoflavone glycitein in water was investigated using the competitive reaction of analyte (free Glyc)and immobilized Glyc-BSA-conjugate with polyclonal antibodies. It was shown that the efficiency to detect Glyc drastically depends on the pH level of the probe solution. With the decrease in pH from 7.4 to 4.0, (i) the affinity of the specific reaction increases and (ii) the level of unspecific sorption becomes saturated. Non-specific adsorption to a SPR sensor surface obscures the specific component and shaded specific response at higher pH (6.0-7.4) when used serum for the quantification of specific analytes. The standard curves obtained in acidic solutions (pH 4-5) indicate that the linear part of the dependence completely covers the range between detection limit (0.1 mug/ml) and Glyc solubility in water (0.9 mug/ml). The difference in SPR- and ELISA-based analytical protocols as well as the requirements for increasing the efficiency in quantitative SPR analysis using purified antibodies is discussed.